Substantiation of technological parameters of the oil extract obtained from Urtica dioica roots based on the yield determination of phytosterols using capillary gas chromatography method

Due to the content of phytosterols, extractive preparations Urtica dioica roots are able show antiandrogenic effect in case external therapy men and women with androgenic alopecia. Oil extracts (OE) characterized by several advantages when applied skin scalp compared water-alcohol extracts. For development OE technology from roots, it is important choose optimal extraction parameters, which based on quantitative determination phytosterols extractant studied samples extracts.
 The aim work parameters for obtaining experimental gas capillary chromatography.
 Materials methods. Objects research – root, refined corn oil, sunflower oil Determination phytosterol was carried out chromatography (chromatograph “Crystal 2000”, manufacturer production company “Analytics”).
 Results. 5 different compounds steroid structure (stigmasterol, ?-sitosterol, etc.) were identified liquid chromatography, 10 (campesterol, 2-? stigmasterol, ?5-avenosterol, oil. ?-sitosterol sum sterols significantly higher this substance amounted 59.33 %. Optimal technological established considering peculiarities amount OE. total plant OE, including their extractant, range 7880 mg/kg; 4638 mg/kg.
 Conclusion. choice UDR yield experimentally substantiated, namely: raw material-extract ratio 1: 5, time 6 h, method maceration